Journal: Cell reports

Article Title: Identification of a Core Amino Acid Motif within the α Subunit of GABA A Rs that Promotes Inhibitory Synaptogenesis and Resilience to Seizures

doi: 10.1016/j.celrep.2019.06.014

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Anti-GABA A R γ2L mouse monoclonal , UC Davis/NIH NeuroMab Facility , Cat #75-442; RRID: AB_2617122.

Techniques: Staining, Recombinant, Saline, Bicinchoninic Acid Protein Assay, Western Blot, Protease Inhibitor, Sequencing, Software, Microscopy

Journal: Journal of Histochemistry & Cytochemistry

Article Title: Expression of CXCL15 (Lungkine) in Murine Gastrointestinal, Urogenital, and Endocrine Organs

doi: 10.1369/jhc.6a7121.2007

Figure Lengend Snippet: Figure 1 Expression of CXCL15 in multiple murine tissues. Top: mRNA expression of CXCL15 in mucosal and endocrine tissues of 12-week-old C57BL/6 mice. Expression is graphed as a ratio of lung CXCL15 expression. Expression was similar for FVB/N and BALB/c mice (data not shown). As previously reported, the lung has a high level of expression of CXCL15, but here we also demonstrate sig- nificant levels of expression in other mucosal and glandular organs including trachea, stomach, jejunum, and adrenal gland. Bottom: CXCL15 protein expression was determined by ELISA. High protein expression is seen in the lung, adrenal gland, and other mucosal tis- sues (n53). *p,0.05 as compared with lung.

Article Snippet: Briefly, a 2-fold excess (on a weight basis) of the recombinant mouse CXCL15 (100 ml of 1 mg/ml; R&D Systems) was preincubated with the biotin goat anti-CXCL15 (1 ml of 50 mg/ml) for 1 hr at 37C.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay

Journal: Journal of Histochemistry & Cytochemistry

Article Title: Expression of CXCL15 (Lungkine) in Murine Gastrointestinal, Urogenital, and Endocrine Organs

doi: 10.1369/jhc.6a7121.2007

Figure Lengend Snippet: Figure 2 Immunofluorescence analysis of CXCL15 protein expression. (A) Expression of CXCL15 (green) in the adrenal gland of a C57BL/6 mouse. Bright staining is seen in the pan-cortex with increased intensity in the zona glomerulosa. All sections were counterstained with the nuclear dye, Hoechst 33258 (blue). (B) CXCL15 (green) immunoreactivity in the trachea of C57BL/6 mouse. (C) CXCL15 (green) can be seen in the colonic goblet cells as indicated by the arrow. (D) Expression of CXCL15 (green) in the uterus of a C57BL/6 mouse. (E) CXCL15 immunoreactivity (green) is seen in the alveolar and bronchoepithelial cells of the lung of a C57BL/6 mouse. Staining was done on a BALB/c and FVB mouse lung fixed in Bouins, and staining was the same as the C57BL/6 mouse lung (data not shown). (F) Anti-CXCL15 immunoreactivity in the lung is blocked by preincubation with CXCL15 protein, demonstrating antibody specificity. Bar 5 50 mm.

Article Snippet: Briefly, a 2-fold excess (on a weight basis) of the recombinant mouse CXCL15 (100 ml of 1 mg/ml; R&D Systems) was preincubated with the biotin goat anti-CXCL15 (1 ml of 50 mg/ml) for 1 hr at 37C.

Techniques: Immunofluorescence, Expressing, Staining

Journal: Journal of Histochemistry & Cytochemistry

Article Title: Expression of CXCL15 (Lungkine) in Murine Gastrointestinal, Urogenital, and Endocrine Organs

doi: 10.1369/jhc.6a7121.2007

Figure Lengend Snippet: Figure 3 CXCL15 expression in gastric prezymogenic cells. (A–C) Identification of surface mucous cells through lectin staining with CTB (red in A) indicates that CXCL15 (green in B) is not expressed in surface mucus cells. (D–F) H1/K1-ATPase-immunoreactive parietal cells (red in D) and CXCL15-immunoreactivity (green in E) do not colocalize (F), indicating that parietal cells do not express CXCL15 protein. (G–I) GSII lectin reactivity (red in G) identifying pure mucus neck cells demonstrates that CXCL15 (green in H) is also not ex- pressed in the main mucus neck cell population. (J–L) Pepsinogen immunoreactivity (red) is seen in a subset of mucus neck and zymo- genic cells (red in J). This staining pattern is similar to CXCL15 (green in K), and expression between pepsinogen and CXCL15 is colocalized in L (yellow). (M–O) Immunolocalization of intrinsic factor (red in M) in zymogenic cells and CXCL15 (green in N) indicates a limited number of cells showing colocalization. (O) Arrow indicates the positive colocalization staining between the two. These results are consistent with expression of CXCL15 in prezymogenic cells that are in the process of differentiating into mature zymogenic cells. Bar 5 50 mm.

Article Snippet: Briefly, a 2-fold excess (on a weight basis) of the recombinant mouse CXCL15 (100 ml of 1 mg/ml; R&D Systems) was preincubated with the biotin goat anti-CXCL15 (1 ml of 50 mg/ml) for 1 hr at 37C.

Techniques: Expressing, Staining

Journal: Journal of Histochemistry & Cytochemistry

Article Title: Expression of CXCL15 (Lungkine) in Murine Gastrointestinal, Urogenital, and Endocrine Organs

doi: 10.1369/jhc.6a7121.2007

Figure Lengend Snippet: Figure 4 Histology and CXCL15 ex- pression in gastric (A,B) and colonic (C–F) models of inflammation. Hema- toxylin/eosin (H/E) staining of C57BL/6 stomachs at 8 weeks after mock (A) or H. felis (B) infection. Note the exten- sive inflammation seen after infec- tion. FVB/N (C) and FVB.mdr1a2/2

Article Snippet: Briefly, a 2-fold excess (on a weight basis) of the recombinant mouse CXCL15 (100 ml of 1 mg/ml; R&D Systems) was preincubated with the biotin goat anti-CXCL15 (1 ml of 50 mg/ml) for 1 hr at 37C.

Techniques: Staining, Infection